Inducible satellite cell depletion attenuates skeletal muscle regrowth following a scald-burn injury

Model System:

Burn

Reference Type:

Journal article

Accession No.:

J81118

Journal:

Journal of Physiology

Year, Volume, Issue, Page(s):

2017 , 595, 21, 6687-6701

Publication Website:

Abstract:

Study examined the time course of satellite cell proliferation and fusion capacities following a scald burn injury and to determine the necessity of satellite cell activity in facilitating the regrowth of burn injury-induced muscle atrophy. To determine the necessity of satellite cells during muscle recovery following a burn injury, a genetically modified mouse model (Pax7CreER-DTA) was used that allows for the conditional depletion of satellite cells in skeletal muscle. Additionally, mice were provided 5-ethynyl-2’-deoxyuridine to determine satellite cell proliferation, activation and fusion. Juvenile satellite cell-wild-type (SC-WT) and satellite cell-depleted (SC-Dep) mice (8 weeks of age) were randomized to sham or burn injury consisting of a dorsal scald burn injury covering 30 percent of total body surface area. Both hindlimb and dorsal muscles were studied at 7, 14 and 21 days post-burn. SC-Dep mice had >93 percent depletion of satellite cells compared to SC-WT. Burn injury induced robust atrophy in muscles located both proximal and distal to the injury site (approximately 30 percent decrease in fiber cross-sectional area). Additionally, burn injury induced skeletal muscle regeneration, satellite cell proliferation and fusion. Depletion of satellite cells impaired post-burn recovery of both muscle fiber cross-sectional area and volume. These findings support an integral role for satellite cells in the etiology of lean tissue recovery following a severe burn injury.

Author(s):