Characterization of adipose-derived stem cells following burn injury

Model System:

Burn

Reference Type:

Journal article

Accession No.:

J81096

Journal:

Stem Cell Reviews and Reports

Year, Volume, Issue, Page(s):

2017 , 13, 6, 781-792

Publication Website:

Abstract:

Study investigated which cells within the adipose tissue contributed toward the production of inflammatory mediators and whether adipose-derived stem cells (ASCs) are affected by the post-burn inflammatory environment. Using a 60-percent rat scald burn model, the authors conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks post burn and from non-injured controls. Endpoints included inflammatory marker expression, expression of ASC-specific cell-surface markers, DNA damage, differentiation potential, and proliferation. Inflammatory marker expression was induced in adipocytes and the SVF at 24 and 48 hours post burn; expression of inflammatory marker mRNA transcripts and protein returned to normal in the SVF isolated 1 week post burn. In enriched ASCs, burns did not alter cell-surface expression of stem cell markers, markers of inflammation, differentiation potential, or proliferative ability. These results suggest that adipocytes and the SVF produce large quantities of inflammatory mediators, but that ASCs do not, after burns and that ASCs are unaffected by burn injury or culturing procedures. The findings also suggest that cells isolated over 48 hours after injury are best for cell culture or tissue engineering purposes.

Author(s):