Adipose-derived stem cells attenuate pulmonary microvascular hyperpermeability after smoke inhalation

Model System:

Burn

Reference Type:

Journal article

Accession No.:

J81100

Journal:

PLoS One

Year, Volume, Issue, Page(s):

2017 , 12, 10, e0185937

Publication Website:

Abstract:

Study tested the hypothesis that intravenously administered adipose-derived stem cells (ASCs) effectively ameliorate the severity of pulmonary microvascular hyper-permeability in ovine models of acute respiratory distress syndrome (ARDS) induced by smoke inhalation. Ten female sheep were surgically prepared. ARDS was induced by cooled cotton smoke inhalation. Following injury, sheep were ventilated, resuscitated with lactated Ringer’s solution, and cardiopulmonary hemodynamics were monitored for 48 hours in a conscious state. Pulmonary microvascular hyper-permeability was assessed by measuring lung lymph flow, extravascular lung water content, protein content in plasma and lung lymph fluid. The sheep were randomly allocated to two groups: (1) ASCs group, infused with 200 million of ASCs in 200mL of PlasmaLyteA starting 1 hour after injury; and (2) control group, treated with 200mL of PlasmaLyteA in a similar pattern. Lung lymph flow increased 9-fold in control sheep as compared to baseline. Protein in the plasma was significantly decreased, while it was increased in the lung lymph. The treatment with ASCs significantly attenuated these changes. Treatment with ASCs almost led to the reversal of increased pulmonary vascular permeability and lung water content. Pulmonary gas exchange was significantly improved by ASCs. Infusion of the ASCs did not negatively affect pulmonary artery pressure and other hemodynamic variables. ASCs infusion was well tolerated. The results suggest that intravenous ASCs modulate pulmonary microvascular hyper-permeability and prevent the onset of ARDS in the experimental model.

Author(s):